Ac-DEVD-pNA
Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (Km=9.7µM) and caspases-1, -4, -6, and -7 [1]. Ac is the acetyl group and pNA is p-nitroanilide. Sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp216 for caspase-3. In apoptosis, activated caspase-3 cleaves substrates such as poly(ADP-ribose) polymerase that targets at the amino acid sequence DEVD [2]. Cleavage is monitored colorimetrically at 405nm. Ac-DEVD-pNA can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
References:
1. Talanian, R.V.,Quinlan, C.,Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).
2. Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994).
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 638.58 |
| Cas No. | 189950-66-1 |
| Formula | C26H34N6O13 |
| Solubility | ≥2.41 mg/mL in EtOH with ultrasonic; ≥6.39 mg/mL in H2O; ≥9.94 mg/mL in DMSO |
| Chemical Name | 7-(2-carboxyethyl)-4-(carboxymethyl)-10-isopropyl-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)CC(O)=O)=O)C(NC(C(C)C)C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
