Ac-VEID-pNA
Ac-VEID-pNA is a colorimetric substrate for caspase-6 (Km=30µM; Kcat/Km=168’000 M-1sec-1), and other similar cysteine proteases, which recognize the amino acid sequence VEID. The sequence is based on the lamin A site of cleavage. During apoptosis, Caspase 6 cleaves the substrate and releases pNA, thus the caspase activity can be calculated by the spectrophotometric detection of pNA at 405nm (ε=10,500 M-1cm-1). Ac-VEID-pNA can be used to identify and quantify caspase-6 activity in apoptotic cell lysates and to study events downstream of caspase-6 activation.
References:
1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.: N.A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997)
2. Substrate specificities of caspase family proteases.: R.V. Talanian et al.; J. Biol. Chem. 272, 9677 (1997)
3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.: S.M. Srinivasula et al.; J. Biol. Chem. 271, 27099 (1996)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 636.65 |
| Cas No. | 189684-54-6 |
| Formula | C28H40N6O11 |
| Solubility | ≥63.7 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 10-(sec-butyl)-7-(2-carboxyethyl)-4-isopropyl-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)C(C)C)=O)C(NC(C(C)CC)C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
