Ac-IETD-pNA
Ac-IETD-pNA is a colorimetric substrate for caspase-8 and other similar cysteine proteases (caspase-6, caspase-10 and granzyme B). It recognizes the amino acid sequence IETD which is based on caspase-8 cleavage site in Caspase-3 precursor. During apoptosis, caspase 8 cleaves the substrate and releases pNA, thus the caspase activity can be calculated by the spectrophotometric detection of pNA at 405nm. Ac-IETD-pNA can be used to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation.
References:
1. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
2. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994)
3. Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 638.62 |
| Cas No. | 219138-21-3 |
| Formula | C27H38N6O12 |
| Solubility | ≥63.9 mg/mL in DMSO; ≥14.03 mg/mL in H2O; ≥5.99 mg/mL in EtOH with ultrasonic |
| Chemical Name | (4S,7S,10S,13S)-4-((S)-sec-butyl)-7-(2-carboxyethyl)-10-((R)-1-hydroxyethyl)-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CC[C@H](NC([C@@H](NC(C)=O)[C@@H](C)CC)=O)C(N[C@@H]([C@H](O)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
Chemical structure
