TCA Deproteinization Sample Preparation Kit

Biological fluids, cell lysates, and tissue lysates often contain high levels of lipids, proteins, and enzymes, which can interfere with the detection of small molecule substances such as amino acids, inorganic ions, and various metabolites. Therefore, protein removal is an essential step in biological sample processing prior to detection.
This product is based on the principle of protein precipitation using trichloroacetic acid (TCA), enabling rapid removal of proteins from biological samples. The operation procedure consists of two steps: first, add TCA to precipitate proteins in the sample and remove the precipitate; then, add a neutralization solution to the supernatant to adjust the sample pH to neutral. The treated supernatant can be directly used for subsequent detection.
This product is suitable for sample pretreatment in the quantitative detection of small molecule substances such as glycogen, adenosine triphosphate (ATP), cyclic adenosine monophosphate (cAMP), glutathione, and various antioxidants. It features established methodology and stable results. This kit is suitable for protein removal operations in both small-scale and large-scale sample processing, and the treated biological samples can be directly used in various biochemical detection assays.