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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Extracted from tea, coffee fruit; Store the product in sealed, cool and dry condition
Cell lines
Patient-derived AC-UPS01 and AC-RMS01 cell lines
Preparation method
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.
Reacting condition
0.25, 0.5, 1, 2.5 and 5 mM; 72 h
Applications
Caffeine significantly inhibited the patient-derived UPS and RMS cell lines in a dose-dependent manner with IC50 values of 2.02 ± 0.22 mM and 2.37 ± 0.48 mM, respectively. Addition of 0.3 mM or 0.6 mM VPA to varying concentrations of CAF enhanced efficacy against both the AC-UPS01 and AC-RMS01 cell lines.
Animal models
diet-induced obesity (DIO) mice
Dosage form
10 μg per mouse, i.c.v. administration
Application
In diet-induced obesity (DIO) mice, administration of caffeine into mouse brain significantly increased the numbers of c-Fos+ cells in the PVN, Arc and DMH nuclei, suggesting that caffeine stimulated the activities of neurons in the hypothalamic nuclei involved in energy balance control. Caffeine reduced the adipocyte sizes of epididymal white adipose tissue, plasma triglycerides (TG) levels and improved glucose tolerance. Mice gained significantly less body weights than the controls on day 7.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1] Igarashi K1,2,3, Kawaguchi K1,2, Kiyuna T1,2, et al. Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines. Anticancer Res. 2017 Aug; 37 (8): 4081-4084.
[2] Wu L1, Meng J1, Shen Q1, et al. Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice. Nat Commun. 2017 Jun 27; 8: 15904.