MitoSOX Red

MitoSOX Red (CAS 1003197-00-9), also referred to as Mito-HE, is a fluorescent probe specifically developed for detecting mitochondrial superoxide in live cell studies. Upon cellular entry, it preferentially accumulates into mitochondria, where it is oxidized exclusively by superoxide radicals, resulting in emission of red fluorescence. Importantly, this reaction is selective, distinguishing mitochondrial superoxide from other reactive oxygen species (ROS) and reactive nitrogen species (RNS). The probe's oxidation can be inhibited by superoxide dismutase (SOD) or superoxide scavengers. Hence, MitoSOX Red serves as a valuable reagent for studying mitochondrial oxidative stress and evaluating superoxide levels in biological research contexts.

MitoSOX Red, also called Mito-HE, is a novel probe for the selective detection of superoxide in the mitochondria of live cells. MitoSOX Red is live-cell permeant that selectively targets the mitochondria. Once in the mitochondria, MitoSOX can be oxidized by superoxide and exhibits bright red fluorescence. MitoSOX is oxidized by superoxide but not by other reactive oxygen species (ROS) or reactive nitrogen species (RNS), indicating that it is superoxide-specific. Meanwhile, oxidation of this probe can be prevented by superoxide dismutase (SOD) as well as superoxide scavengers.