Amplex Red Catalase Activity Assay Kit

Catalase is an enzyme that is ubiquitous in cells, mainly found in the liver, kidneys, and red blood cells. Its main function is to catalyze the decomposition of hydrogen peroxide (H₂O₂) to produce water and oxygen, thereby effectively reducing the concentration of H₂O₂ in cells. H₂O₂ is a strong oxidant that can trigger oxidative stress, damage cells and tissues, and lead to cell dysfunction and the occurrence of a variety of diseases. Therefore, catalase plays an important role in maintaining the stability of the intracellular environment and protecting cells from oxidative damage. Studying the activity of catalase and its regulatory mechanism is of great significance for understanding the antioxidant defense mechanism of cells and exploring the occurrence and development of diseases related to oxidative stress.

Amplex Red Catalase Activity Assay Kit provides a sensitive and simple method for measuring catalase activity. The working principle of this kit is based on catalase-catalyzed decomposition of H₂O₂. Catalase catalyzes H₂O₂ into water and oxygen (O₂). Under the presence of horseradish catalase (HRP), the remaining unreacted H₂O₂ can react with the Amplex Red reagent to form highly fluorescent resorufin. Therefore, as catalase activity increases, the fluorescence signal of resorufin decreases. Catalase activity in the sample can be indirectly detected by setting a no-catalase control. >

Figure 1: Reference Catalase standard curve