Lipid Peroxidation (MDA) Assay Kit Plus
Malondialdehyde (MDA) is one of the products of lipid peroxidation in organisms, and it is widely used as a detection indicator for lipid peroxidation.
Lipid Peroxidation (MDA) Assay Kit Plus is a kit for the detection of MDA in samples such as tissues, cell lysates, plasma, serum, and urine by thiobarbituric acid (TBA). The detection principle is that MDA reacts with TBA to produce a red product that is specifically absorbed at 535 nm and can therefore be determined by colorimetry. At the same time, the reaction products can also be excited at 535 nm, resulting in a maximum emission wavelength of 553 nm, so fluorescence detection can also be performed.
The kit provides antioxidants that can inhibit the production of new MDA in the sample during the detection process, making the detection more accurate. At the same time, the kit can detect MDA as low as 1 μM and has a good linear relationship in 1-200 μM. Compared to Lipid Peroxidation (MDA) Assay Kit (Cat. No: K2167), this kit provides an MDA lysis buffer, eliminating the need for separate purchase and offering greater convenience in use.
Figure 1: Reference standard curve of this kit
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Components |
100 Assays |
500 Assays |
Storage |
|
Lysis Buffer |
20 mL |
100 mL |
-20°C |
|
TBA |
25 mg |
125 mg |
-20°C away from light |
|
TBA Preparation Buffer |
7 mL |
35 mL |
-20°C |
|
TBA Dilution Buffer |
15 mL |
75 mL |
-20°C |
|
Antioxidant |
300 μL |
1.5 mL |
-20°C away from light |
|
MDA Standard (1 mM) |
200 μL |
1 mL |
-20°C |
|
Shipping: Blue ice Shelf life: 1 year |
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