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Fluo-4 AM

Catalog No.
B8807
A fluorescent Calcium probe, cell permeant
Grouped product items
SizePriceStock Qty
20ul
$92.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Fluo-4 AM (CAS: 273221-67-3) is a fluorescent calcium indicator widely utilized in biomedical research for intracellular calcium concentration measurement. This acetoxymethyl ester derivative readily diffuses across cell membranes and is hydrolyzed intracellularly by esterases, generating the calcium-sensitive fluorescent dye Fluo-4. Binding of Fluo-4 to cytosolic Ca2? ions induces a significant increase in fluorescence intensity, enabling precise real-time calcium monitoring. Structurally derived from Fluo-3 AM by substituting chlorine with fluorine, Fluo-4 AM exhibits quicker loading kinetics and approximately doubled fluorescence intensity upon excitation at 488 nm. It finds extensive application in cell signaling, functional assays, and pharmacological assessments of calcium-dependent processes.

Description

Fluo-4 AM is one of the most common probes for detecting Ca2+. Fluo-4 AM is an acetoxymethyl (AM) ester derivative of Fluo-4 that can penetrate cell membranes and be cleaved by intracellular esterase to form Fluo-4. Fluo-4 can remain in cells and bind to intracellular Ca2+, then emit bright fluorescence. Fluo-4 AM is an analog of Fluo-3 AM with two chlorine substituents replaced by fluorines. This modification results in increased fluorescence excitation at 488 nm and a greater signal level for the image. In addition, the fluorescence produced by Fluo-4 binding Ca2+ is at least 100 folds stronger than UV-excited probes (Fura-2 and Indo-1). This reagent is provided as a 2 mM stock solution in DMSO.

Properties

Ex/Em 494/516 (after binding Ca2+)
Storage The product should be stored at -20°C away from light and moisture.
Shipping Condition Blue ice
EXP Date This product should be stored at -20°C away from light and moisture. Avoid repeated freeze/thaw cycles, stable for 6 months.

Chemical Properties

Physical AppearanceLiquid
M.Wt1096.95
Cas No.273221-67-3
FormulaC51H50F2N2O23
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & DataSheet

View current batch:
 

Components and Storage

Components B8807-20 µL
Fluo-4 AM 20 µL (2 mM)

This product should be stored at -20°C away from light and moisture. Avoid repeated freeze/thaw cycles, stable for 6 months.