Fluo-4 AM is one of the most common probes for detecting Ca2+. Fluo-4 AM is an acetoxymethyl (AM) ester derivative of Fluo-4 that can penetrate cell membranes and be cleaved by intracellular esterase to form Fluo-4. Fluo-4 can remain in cells and bind to intracellular Ca2+, then emit bright fluorescence. Fluo-4 AM is an analog of Fluo-3 AM with two chlorine substituents replaced by fluorines. This modification results in increased fluorescence excitation at 488 nm and a greater signal level for the image. In addition, the fluorescence produced by Fluo-4 binding Ca2+ is at least 100 folds stronger than UV-excited probes (Fura-2 and Indo-1). This reagent is provided as a 2 mM stock solution in DMSO.
Properties
Ex/Em
494/516 (after binding Ca2+)
Storage
The product should be stored at -20°C away from light and moisture.
Shipping Condition
Blue ice
EXP Date
This product should be stored at -20°C away from light and moisture. Avoid repeated freeze/thaw cycles, stable for 6 months.
Chemical Properties
Physical Appearance
Liquid
M.Wt
1096.95
Cas No.
273221-67-3
Formula
C51H50F2N2O23
Shipping Condition
Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips
We do not recommend long-term storage for the solution, please use it up soon.
