Fluo-4 AM (CAS: 273221-67-3) is a fluorescent calcium indicator widely utilized in biomedical research for intracellular calcium concentration measurement. This acetoxymethyl ester derivative readily diffuses across cell membranes and is hydrolyzed intracellularly by esterases, generating the calcium-sensitive fluorescent dye Fluo-4. Binding of Fluo-4 to cytosolic Ca2? ions induces a significant increase in fluorescence intensity, enabling precise real-time calcium monitoring. Structurally derived from Fluo-3 AM by substituting chlorine with fluorine, Fluo-4 AM exhibits quicker loading kinetics and approximately doubled fluorescence intensity upon excitation at 488 nm. It finds extensive application in cell signaling, functional assays, and pharmacological assessments of calcium-dependent processes.
Properties
Ex/Em
494/516 (after binding Ca2+)
Storage
The product should be stored at -20°C away from light and moisture. Please use Low Binding tubes for aliquots.
Shipping Condition
Blue ice
EXP Date
This product should be stored at -20°C away from light and moisture. Avoid repeated freeze/thaw cycles, stable for 6 months.
Chemical Properties
Physical Appearance
Liquid
M.Wt
1096.95
Cas No.
273221-67-3
Formula
C51H50F2N2O23
Shipping Condition
Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips
We do not recommend long-term storage for the solution, please use it up soon.
