Fluo-4 AM Calcium Assay Kit

Fluo-4 AM is one of the most common probes for detecting Ca²⁺. Fluo-4 AM is an acetoxymethyl (AM) ester derivative of Fluo-4 that can penetrate cell membranes and be cleaved by intracellular esterase to form Fluo-4. Fluo-4 can remain in cells and bind to intracellular Ca²⁺, then emit bright fluorescence. Fluo-4 AM is an analog of Fluo-3 AM with two chlorine substituents replaced by fluorines. This modification results in increased fluorescence excitation at 488 nm and a greater signal level for the image. In addition, the fluorescence produced by Fluo-4 binding Ca²⁺ is at least 100 folds stronger than UV-excited probes (Fura-2 and Indo-1).

Fluo-4 AM Calcium Assay Kit is a kit for the detection of intracellular calcium concentration based on the Fluo-4 AM probe. The kit is optimized for easy to use and can be detected without washing after staining. The Assay Buffer provided in this kit can ensure the viability of the cells. The kit also provides the Solubility Enhancer and Staining Enhancer, which can be used to enhance the solubility of the probe and to reduce background fluorescence. This kit is ideal for large-scale calcium detection and screening of GPCR inhibitors or agonists.

Figure 1: This kit is used to detect calcium signal in HeLa cells