Dihydrotestosterone(DHT)
Dihydrotestosterone is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor.
In AR-positive bladder cancer UMUC3 and TCC-SUP cells, dihydrotestosterone (DHT) increases the expression of EGFR and ERBB2 both in mRNA and in protein levels. In AR-positive cell，DHT additionally upregulates the levels of phosphorylation of EGFR (pEGFR) and its downstream proteins AKT (pAKT) and ERK1/2 (pERK), induced by EGF treatment [1].
DHT-treated SOD1-G93A mice demonstrate ameliorated muscle atrophy and increased body weight, which is associated with stronger grip-strength. DHT treatment increases the expression of insulin-like growth factor-1 in muscle, which can exert myotrophic as well as neurotrophic effects through retrograde transport. DHT treatment attenuates neuromuscular junction denervation, and axonal and motoneuron loss. DHT-treated SOD1-G93A mice demonstrates improvement in motor behavior as assessed by rota-rod and gait analyses, and an increased lifespan [2].
References:
[1] Zheng Y, Izumi K, Yao J L, et al. Dihydrotestosterone upregulates the expression of epidermal growth factor receptor and ERBB2 in androgen receptor-positive bladder cancer cells. Endocrine Related Cancer, 2011, 18 (4): 451-464.
[2] Young-Eun Y, Chien-Ping K, Lin M. Dihydrotestosterone Ameliorates Degeneration in Muscle, Axons and Motoneurons and Improves Motor Function in Amyotrophic Lateral Sclerosis Model Mice. PLoS ONE, 2012, 7 (5): e37258-.
- 1. Yu Tong, Yi-Jun Guo, et al. "Combined treatment with dihydrotestosterone and lipopolysaccharide modulates prostate homeostasis by upregulating TNF-α from M1 macrophages and promotes proliferation of prostate stromal cells." Asian J Androl. 2022 Sep-Oct;24(5):513-520. PMID: 34975070
- 2. YI HE, Wasti Binaya, et al. "Androgen combination estrogen improve asthma by mediating Runx3 expression." Research Square. August 2nd, 2022.
- 3. Chunjie Liu, Yongfen Lyu, et al. "A hemizygous mutation in the androgen receptor gene causes different phenotypes of androgen insensitivity syndrome in two siblings by disrupting the nuclear translocation." Mol Genet Genomics. 2020 Sep;295(5):1103-1111. PMID: 32435981
- 4. Yu Zhang, Jing Zhao, et al. "Loss of Exosomal miR-146a-5p from CancerAssociated Fibroblasts After Androgen Deprivation Therapy Contributes to Prostate Cancer Metastasis." Research Square. 17 Jul, 2020
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 290.44 |
| Cas No. | 521-18-6 |
| Formula | C19H30O2 |
| Solubility | ≥29 mg/mL in DMSO; insoluble in H2O; ≥13.6 mg/mL in EtOH |
| Chemical Name | (5S, 8R, 9S, 10S, 13S, 14S, 17S)-17-hydroxy-10, 13-dimethyltetradecahydro-1H-cyclopenta[a]phenanthren-3(2H)-one |
| SDF | Download SDF |
| Canonical SMILES | C[C@]12CC[C@H]3[C@@H](CC[C@H]4CC(=O)CC[C@]34C)[C@@H]1CC[C@@H]2O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Androgen receptor-positive bladder cancer UMUC3 and TCC-SUP cells |
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Reaction Conditions |
1 or 10 nM dihydrotestosterone for 24 h incubation |
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Applications |
Dihydrotestosterone treatment at 1 nM for 24 h increased EGFR levels to 1.7- and 1.9-fold in UMUC3 and TCC-SUP cell lines respectively, compared with mock treatment. Similarly, dihydrotestosterone treatment resulted in up to 1.7-fold increase in ERBB2 levels in both cell lines. Dihydrotestosterone was found to increase EGFR and ERBB2 transcript abundance in both cell lines in a dose-dependent manner. |
| Animal experiment:[2] | |
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Animal models |
SOD1-G93A mice |
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Dosage form |
An estimated plasma concentration of ~ 500 ng/dl Administered in the form of silastic implant |
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Applications |
Dihydrotestosterone treatment ameliorated degeneration in muscle, axons and motoneurons, as well as improved motor function in amyotrophic lateral sclerosis (ALS) model mice. Application of dihydrotestosterone was a relatively simple and non-invasive procedure, which could be translated into therapy to improve the quality of life for ALS patients. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Zheng Y, Izumi K, Yao JL, et al. Dihydrotestosterone upregulates the expression of epidermal growth factor receptor and ERBB2 in androgen receptor-positive bladder cancer cells. Endocrine-Related Cancer, 2011, 18(4): 451-464. 2. Yoo YE, Ko CP. Dihydrotestosterone ameliorates degeneration in muscle, axons and motoneurons and improves motor function in amyotrophic lateral sclerosis model mice. PLoS One, 2012, 7(5): e37258. |
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