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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cy7 azide is a labeling reagent that ready for the use in Click Chemistry labeling. This labeling dye is a near-infrared dye which has low aqueous solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecules is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. This product can be used for the incorporation of Cy7 azide into various biomolecules and post-synthetic modification of oligonucleotides is also available.
PEG-b-PPLG-Cy7 was conjugated by Cy7 azid and the polymer of PEG-b-PPLG. In blank micelles, it was used to probe the association of unimers [1].
Reference:[1] Morton, S. W.; Zhao, X.; Quadir, M.A.; Hammond, P.T. FRET-enabled biological characterization of polymeric micelles. Biomaterials,2014, 35(11), 3489-3496.