L-Carnitine Colorimetric/Fluorometric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Carnitine is a quaternary ammonium compound produced from methionine and lysine. Carnitine plays an important role in transporting fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where occurs β-oxidation, acetate is produced and is utilized to generate energy in the TCA cycle. Carnitine exists in two isomers. Only L-carnitine is biologically active. L-Carnitine is often considered as a nutritional supplement.
The L-Carnitine Colorimetric/Fluorometric Assay Kit provides a simple, fast and convenient way for detection of free L-carnitine in various biological samples based on colorimetric and fluorometric method. In the assay, CoA transfers an acetyl group to carnitine and the free CoA produced is further processed with subsequent oxidation of the Oxi-Red probe to give absorbance (570 nm) and fluorescence (Ex/Em 535 nm 587 nm). The normal range of L-carnitine in serum is ~20-100 µM. The detection sensitivity is ~10 µM for the colorimetric assay and ~1 µM for the fluorometric assay.
Features & Properties
|Features||Fast and convenient Simple procedure; takes ~ 30 minutes. Kit contains all necessary reagents for accurate measurement of L-carnitine levels.|
|Storage Conditions||Store at -20℃.|
Quality Control & DataSheet
- View current batch:
|Kit components||Carnitine Assay Buffer Carnitine Probe (in DMSO) Carnitine Converting Enzyme Carnitine Substrate Mix Carnitine Development Mix Carnitine Standard (10 µmol)|