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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein (MAP) kinase family identified in mammals. Activation of JNK plays an critical role in neuronal apoptosis and other physiological and pathological processes.
The KinaseSTAR JNK Activity Screening Kit provides a simple and convenient way for screening of JNK activity based on Western Blot method. The assay uses an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell or tissue lysates. After washing to remove nonspecifically bound proteins, the kinase reaction is conduced in the presence of cold ATP. The phosphorylation of c-Jun is detected by Western blot method using a phospho-c-Jun specific antibody. The kit is suited for both research and drug discovery.
