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Hydroxyproline Colorimetric Assay Kit

Catalog No.
K2083
Detects hydroxyproline, sensitive.
Grouped product items
SizePriceStock Qty
100 assays
$474.00
In stock

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Background

Hydroxyproline is a common nonproteinogenic amino acid. In mammals, hydroxyproline is found only in elastin and collagen. However, it exists in a variety of other proteins in plants. Hydroxyproline is formed by proline hydroxylase in the peptide chain as a post-translational modification and can not be formed from free proline. A variety of diseases such as neoplastic, renal or bone disease, inflammatory, endocrine and autoimmune disorders affect collagen turnover and cause elevated urine and serum hydroxyproline. Hydroxyproline hydrolysates in tissue can be used for the measurement of the amount of collagen or gelatin present.

 The Hydroxyproline Colorimetric Assay Kit provides a sensitive, simple and convenient way for detection of hydroxyproline in tissue or protein/peptide hydrolysates based on colorimetric method. The assay can also be used for measurement of hydroxyproline content in other biological samples such as serum or urine with a prior purification process. The result can be easily qualified using a microtiter plate reader or a spectrophotometer at 560 nm. The assay is useful over the range of 0.1-2 μg.

Features & Properties

FeaturesConvenient and easy to use. Simple procedure; takes less than ~100 minutes.
ShippingGel pack
Storage ConditionsStore at +4℃.

Quality Control

Quality Control & DataSheet

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Related Biological Data

Hydroxyproline Colorimetric Assay Kit

Related Biological Data

Hydroxyproline Colorimetric Assay Kit

Related Biological Data

Hydroxyproline Colorimetric Assay Kit

Related Biological Data

Hydroxyproline Colorimetric Assay Kit
 

Overview

Kit componentsOxidation Buffer Chloramine T Concentrate Perchloric acid/Isopropanol Solution DMAB Concentrate (in DMSO) Hydroxyproline Standard (1 mg/ml)