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3-Methyladenine

Catalog No.
A8353
Class III PI3K inhibitor
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$50.00
In stock
50mg
$50.00
In stock
200mg
$120.00
In stock
500mg
$240.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

3-Methyladenine is an inhibitor of class III phosphoinositide 3-kinase (PI3K) [1].

3-Methyladenine is widely used as an autophagy inhibitor through inhibiting class III PI3K without significantly affecting protein synthesis or ATP levels. 3-Methyladenine also inhibits class I PI3K. It is found that 3-Methyladenine has different effects on class I and class III PI3K. It blocks class I PI3K persistently while inhibits class III PI3K transiently. As an inhibitor of autophagy, 3-Methyladenine is usually used to study the role of autophagy. In addition, 3-Methyladenine is also found to have anti-cancer efficacy. It induces cell death of tumor under nutrient-starved conditions. In HT1080 cells, 3-Methyladenine significantly inhibits cell migration through reducing membrane ruffle and lamellipodia formation in normal culture condition. It also inhibits the invasion of HT1080 cells [1, 2].

References:
[1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
[2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt149.15
Cas No.5142-23-4
FormulaC6H7N5
Synonyms3-MA
Solubility≥5 mg/mL in H2O; ≥7.45 mg/mL in DMSO; ≥8.97 mg/mL in EtOH
Chemical Name3-methylpurin-6-amine
SDFDownload SDF
Canonical SMILESCN1C=NC(=C2C1=NC=N2)N
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:

Cell lines

HT1080 cells

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

5 or 10 mM; 10 hrs

Applications

3-Methyladenine (3-MA) (5 ~ 10 mM) strongly inhibited HT1080 cell migration. In addition, 10 mM 3-MA showed a stronger effect on the suppression of HT1080 cell migration than both 100 nM WMN and 25 μM LY294002.

References:

[1]. Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.

Biological Activity

Description 3-Methyladenine is a selective inhibitor of PI3K with an IC50 values of 25 μM and 60 μM for Vps34 and PI3Kγ, respectively.
Targets Vps34 PI3Kγ        
IC50 25 μM 60 μM        

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