3-Methyladenine
Catalog No.
A8353
Class III PI3K inhibitor
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3-Methyladenine is an inhibitor of class III phosphoinositide 3-kinase (PI3K) [1].
3-Methyladenine is widely used as an autophagy inhibitor through inhibiting class III PI3K without significantly affecting protein synthesis or ATP levels. 3-Methyladenine also inhibits class I PI3K. It is found that 3-Methyladenine has different effects on class I and class III PI3K. It blocks class I PI3K persistently while inhibits class III PI3K transiently. As an inhibitor of autophagy, 3-Methyladenine is usually used to study the role of autophagy. In addition, 3-Methyladenine is also found to have anti-cancer efficacy. It induces cell death of tumor under nutrient-starved conditions. In HT1080 cells, 3-Methyladenine significantly inhibits cell migration through reducing membrane ruffle and lamellipodia formation in normal culture condition. It also inhibits the invasion of HT1080 cells [1, 2].
References:
[1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
[2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 149.15 |
| Cas No. | 5142-23-4 |
| Formula | C6H7N5 |
| Synonyms | 3-MA |
| Solubility | ≥5 mg/mL in H2O; ≥7.45 mg/mL in DMSO; ≥8.97 mg/mL in EtOH |
| Chemical Name | 3-methylpurin-6-amine |
| Canonical SMILES | CN1C2=NC=NC2=C(N)N=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
HT1080 cells |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
5 or 10 mM; 10 hrs |
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Applications |
3-Methyladenine (3-MA) (5 ~ 10 mM) strongly inhibited HT1080 cell migration. In addition, 10 mM 3-MA showed a stronger effect on the suppression of HT1080 cell migration than both 100 nM WMN and 25 μM LY294002. |
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References: [1]. Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268. |
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| Description | 3-Methyladenine is a selective inhibitor of PI3K with an IC50 values of 25 μM and 60 μM for Vps34 and PI3Kγ, respectively. | |||||
| Targets | Vps34 | PI3Kγ | ||||
| IC50 | 25 μM | 60 μM | ||||
Quality Control & MSDS
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