2X RNA Loading Buffer

2X RNA Loading Buffer is a modified, 2X concentrated RNA loading buffer. It can be used for both denaturing and non-denaturing agarose gel RNA electrophoresis, as well as polyacrylamide gel RNA electrophoresis. Its main components include SDS, EDTA, bromophenol blue, and xylene cyanol, and it is free of RNase contamination. Bromophenol blue and xylene cyanol serve as tracking dyes during electrophoresis, indicating the progress of the electrophoresis.

Nucleic acid stains can be added directly to the gel before electrophoresis, or the gel can be incubated in a nucleic acid staining solution after electrophoresis and then imaged. Recommended nucleic acid stains include Safe DNA Gel Stain (Catalog No. A8743) and Hyper Gel Red (Catalog No. A8746).