10-methyl-9-(phenoxycarbonyl) Acridinium fluorosulfonate

10-methyl-9-(phenoxycarbonyl) Acridinium fluorosulfonate (CAS No.: 149300-54-9) is a phenyl ester derivative of acridinium-based chemiluminescent probes widely employed in biochemical and analytical research as a highly sensitive fluorescent dye for oxidative species detection. This compound belongs to the acridinium ester class, which is characterized by its ability to undergo rapid chemiluminescent reactions under mild, near-neutral conditions, typically initiated by reactive oxygen species such as hydrogen peroxide, leading to photon emission through a well-defined oxidative decomposition mechanism. It is therefore commonly utilized in assays designed to quantify hydrogen peroxide and related reactive intermediates, serving as an indirect reporter of oxidative stress, enzymatic activity, or redox signaling pathways in vitro. Owing to its high signal-to-noise ratio, rapid kinetics, and stable luminescent output, it is frequently incorporated into bioanalytical platforms including immunoassays, enzyme-linked detection systems, and high-throughput screening workflows relevant to drug discovery and molecular diagnostics research. While specific potency metrics are not typically defined for this class of luminescent reagents, their functional performance is generally observed across low micromolar to nanomolar working concentrations depending on assay configuration. In experimental settings, the concentrations employed are determined by assay sensitivity requirements and system optimization parameters, and its application spans cell-free biochemical assays as well as complex biological samples for monitoring oxidative processes and evaluating enzyme-catalyzed reactions.