WST-8 Glucose Uptake Assay Kit
Glucose metabolism is a process that converts ingested glucose into energy, which is the main source of energy supply in living organisms. Studies have shown that glucose metabolism is closely related to many diseases such as cancer, diabetes and obesity, so it is important to monitor glucose uptake.
The WST-8 Glucose Uptake Assay Kit is a kit for detecting glucose uptake based on the WST-8 chromogenic reaction. The glucose analogue 2-DG can be uptaken into cells by glucose transporters and converted to 2-DG6P by endogenous enzymes. G6PDH catalyzes 2-DG6P into 6PGL, as well as the conversion of NADH+ to NADPH. NADPH can react with WST-8 to yield an orange-yellow formazan with a maximum absorption peak at 450 nm. Its absorbance is directly proportional to the ability of glucose uptake.
This kit can sensitively, rapidly and non-radioactively detect glucose uptake. The kit has a good linearity at concentrations of 10-500 μM.
Figure 1: Typical standard curve and assay data. (A) The reference standard curve, (B) the glucose uptake capacity of HeLa cells after oleic acid induction. Insulin(I), which is used to enhance glucose uptake; Phloretin(P), which is used to inhibit glucose uptake.
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Components |
100 Assays |
500 Assays |
Storage |
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Glucose Uptake Lysis Buffer |
15 mL |
70 mL |
-20°C |
|
Glucose Uptake Assay Buffer |
10 mL |
50 mL |
-20°C |
|
KRPH Buffer |
12 mL |
60 mL |
-20°C |
|
Glucose Uptake Reagent A |
200 μL |
1 mL |
-20°C away from light |
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Glucose Uptake Reagent B |
100 μL |
500 μL |
-20°C |
|
Enzyme Solution |
100 μL |
500 μL |
-20°C |
|
2-DG (10 mM) |
1 mL |
5 mL |
-20°C |
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2-DG6P Standard (10 mM) |
50 μL |
250 μL |
-20°C |
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Shipping: Blue ice Shelf life: 1 year |
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