Lactate Dehydrogenase Activity Assay Kit (WST-8)

Lactate dehydrogenase (LDH) is a key metabolic enzyme widely present in living cells, which can catalyze the reversible conversion of lactate to pyruvate, as it converts NAD⁺ to NADH. When cells are damaged, LDH is released into the blood or body fluids, so it is often considered an important biomarker for evaluating tissue damage (e.g., myocardial infarction, hepatitis, tumors). The detection of LDH activity is of great significance for clinical disease diagnosis.

This kit provides a quickly, convenient and sensitive assay to detect LDH activity in serum, tissue, cell and other samples. In this assay, LDH catalyzes lactate to produce pyruvate and reduces NAD⁺ to NADH, which can react with WST-8 to produce orange-yellow formazan. Formazan has a specific absorption at 450 nm and its absorbance is proportional with LDH enzyme activity.

Figure 1: Typical standard curve and assay data. (A) NADH standard curve, (B) LDH activity in different samples.