PolQi1
PolQi1 (CAS 2607139-80-8) is a selective small-molecule inhibitor targeting the polymerase domain of DNA polymerase θ (Polθ). Its primary function is to block the alternative end-joining (alt-EJ/TMEJ) pathway. PolQi1 is widely used to optimize genome editing mediated by CRISPR/Cas9 and Prime Editing (PEn), reducing imprecise repair and enhancing targeted integration efficiency.
References:
[1] Wimberger S, Akrap N, Firth M, Brengdahl J, Engberg S, Schwinn MK, Slater MR, Lundin A, Hsieh PP, Li S, Cerboni S, Sumner J, Bestas B, Schiffthaler B, Magnusson B, Di Castro S, Iyer P, Bohlooly-Y M, Machleidt T, Rees S, Engkvist O, Norris T, Cadogan EB, Forment JV, Šviković S, Akcakaya P, Taheri-Ghahfarokhi A, Maresca M. Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing. Nat Commun. 2023 Aug 14;14(1):4761. doi: 10.1038/s41467-023-40344-4. PMID: 37580318; PMCID: PMC10425386.
[2] Antoniou Panagiotis, et al. Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineering. BioRxiv (2024): 2024-02.
| Storage | Store at -20°C |
| M.Wt | 448.77 |
| Cas No. | 2607139-80-8 |
| Formula | C18H14ClF5N4O2 |
| Synonyms | Example 158; 4-Imidazolidinecarboxamide; (4S)-PolQi1 |
| Chemical Name | (S)-N-(3-chloro-2,4-difluorophenyl)-N-methyl-3-(6-methyl-4-(trifluoromethyl)pyridin-2-yl)-2-oxoimidazolidine-4-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | O=C([C@@H]1CNC(N1C2=CC(C(F)(F)F)=CC(C)=N2)=O)N(C)C3=C(F)C(Cl)=C(F)C=C3 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
