Bst DNA Polymerase, Large Fragment
Bst DNA Polymerase, Large Fragment, the large fragment of DNA Polymerase I from Bacillus stearothermophilus (Bst), is obtained by recombinant expression in Escherichia coli. Bst DNA Polymerase, Large Fragment possesses 5'→3' DNA polymerase activity and strong strand displacement activity, while it lacks 5'→3' exonuclease activity due to deletion mutation.
The product is widely applied in nucleic acid isothermal amplification reactions, including loop-mediated isothermal amplification (LAMP) and rolling-circle amplification (RCA). It is also suitable for sequencing of high-GC content DNA, rapid sequencing of nanogram-level DNA templates, library construction and sequencing, and other related applications.
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Components |
800 U |
4000 U |
Storage |
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Bst DNA Polymerase, Large Fragment (8 U/μL) |
100 μL |
500 μL |
-20 °C |
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10 × Bst Reaction Buffer |
300 μL |
1.5 mL |
-20 °C |
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100 mM MgSO4 |
200 μL |
1 mL |
-20 °C |
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Shipping: Dry Ice Shelf life: 12 months |
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