phi29 DNA polymerase
Phi29 DNA Polymerase is recombinantly expressed in Escherichia coli and originates from Bacillus subtilis bacteriophage phi29, with a molecular weight of approximately 67 kDa. Possessing strong processive DNA synthesis capability, phi29 DNA Polymerase can continuously synthesize and extend DNA fragments over 70 kb in a single polymerization reaction, making it suitable for stable whole-genome amplification.
With intrinsic 3'→5' exonuclease activity, phi29 DNA Polymerase ensures high fidelity in amplification reactions. It is widely applied in heat-cycle independent high-fidelity isothermal PCR, rolling circle replication, multiple displacement amplification (MDA), as well as whole-genome amplification of single cells, pathogenic microorganisms and metagenomes.
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Components |
250 U |
1000 U |
5000 U |
Storage |
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phi29 DNA Polymerase (10 U/μL) |
25 μL |
100 μL |
500 μL |
-20 °C |
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10 × phi29 Reaction Buffer |
100 μL |
300 μL |
1.5 mL |
-20 °C |
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Shipping: Dry Ice Shelf life: 12 months |
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