N-2 Supplement (100×)

N-2 Supplement (100×) is a serum-free, chemically defined additive designed for the culture of neural cell lines. Its main components include human transferrin, bovine insulin, progesterone, putrescine, and sodium selenite. N-2 Supplement (100×) supports the growth and expression of neuroblastoma, peripheral nervous system (PNS)-derived, and central nervous system (CNS)-derived post-mitotic neurons. It is commonly used for culturing neural cell lines, as well as for the maintenance and differentiation of pluripotent stem cells. This product is compatible with a wide range of cell culture media, especially serum?free media or DMEM/F12 supplemented with growth factors such as bFGF and EGF.

The supplement is supplied as a sterile-filtered, ready-to-use solution in cell-culture-grade water.