ITS-X Media Supplement (100×)

ITS-X Media Supplement (100×) – fully named Insulin-Transferrin-Selenium-Ethanolamine Media Supplement (100×) – is a cell culture medium additive. It significantly reduces dependence on fetal bovine serum (FBS) by supplying essential components such as insulin, transferrin, selenium, and ethanolamine， and is commonly employed in serum-free or low?serum culture systems. The use of this supplement typically allows fetal bovine serum concentration to be lowered from 10% to 2-4%.

The insulin included in this product promotes cell metabolism and proliferation, transferrin supplies essential iron, selenium exerts antioxidant effects, and ethanolamine serves as a precursor of phosphoglycerides, helping to maintain the structure of plasma membranes and organelles. We offer three ITS media supplements. Their differences are listed in the table below.

This product is prepared in phenol red?free Earle’s Balanced Salt Solution (EBSS), which is sterile?filtered and ready for direct use in cell culture.