HyperFluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody
|
Name |
HyperFluor™ 555 Goat Anti-Rabbit IgG (H+L) Antibody |
|
Host species |
Goat |
|
Target species |
Rabbit |
|
Tested applications |
WB, ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISA |
|
Conjugation |
HyperFluor™ 555 |
|
Ex/Em |
555 nm / 565 nm |
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for Rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
- 1. Ke Zhao, Yongzhi Cui, et al. "Exercise Mimetic Exosomes Re-establish the Extracellular Matrix Metabolic Balance and Alleviate the Inflammatory Macrophage Infiltration in Intervertebral Disc Degeneration." Adv Healthc Mater. 2025 May 19:e2500219
- 2. Jingxin Shan, Zhiyuan Kong, et al. "Formation of Stable Vascular Networks by 3D Coaxial Printing and Schiff-Based Reaction." Gels. 2024 May 25;10(6):366. PMID: 38920913
|
Application |
Notes |
|
ICC/IF |
1/500 - 1/2000 |
|
IHC-Fr |
Use at an assay dependent dilution. |
|
IHC-P |
1/100 - 1/500 |
|
Flow Cyt |
1/250 - 1/1000 |
|
ELISA |
Use at an assay dependent dilution. |
|
WB |
1/5000 - 1/10000 |
|
Form |
Liquid |
|
Storage buffer |
50% Glycerol，1% BSA，PBS，0.02% NaN3 |
|
Storage instructions |
Shipped at 4°C. Store at 4°C short term (2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Keep away from Light. |
|
Concentration |
1 mg/mL |
|
Purity |
Immunogen affinity purified |
|
Purification notes |
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. |
|
Clonality |
Polyclonal |
|
lsotype |
IgG |