ER-Tracker Green

ER-Tracker Green (CAS 730931-46-1) is a green fluorescent probe used specifically for visualizing the endoplasmic reticulum (ER) in living cells. Chemically, it consists of glibenclamide conjugated to the fluorophore BODIPY FL. Glibenclamide targets the sulfonylurea receptors of ATP-sensitive potassium channels localized on the ER membrane, enabling selective fluorescent labeling. ER-Tracker Green exhibits reduced cellular toxicity and enhanced ER specificity compared to traditional dyes such as DiOC?(3), showing minimal mitochondrial labeling. It is suitable for live-cell ER staining but not recommended for fixed-sample applications.

ER-Tracker Green is a green-fluorescent endoplasmic reticulum (ER) probe that selectively labels ER of live cells. ER-Tracker Green is the BODIPY FL labeled glibenclamide. Glibenclamide, a drug for diabetic patients, specially binds to the sulphonylurea receptors of ATP-sensitive K⁺ channels on ER. So fluorescent-labeled glibenclamide (ER-Tracker Green) can label ER. Unlike conventional ER probe DiOC₆(3), ER-Tracker Green does not appear to be toxic to cells at low concentrations, while it is highly selective for ER and rarely stains mitochondria. ER-Tracker Green stains live cells well but is not well-retained after fixation with formaldehyde. Glibenclamide may affect ER function, and variable expression of sulphonylurea receptors in some special cell types may lead to non-ER labeling.

This product is supplied as a lyophilized powder. Dissolve a vial in 20 μL of DMSO to make 1 mM ER-Tracker Green Stock Solution.