DNase I (GMP-grade)
DNase I is a deoxyribonucleic acid endonuclease that hydrolyzes single- or double-stranded DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 3’-hydroxyl and 5’-phosphate group ends. The activity of DNase I is Ca2+ dependent and can be activated by either Mg2+ or Mn2+. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA. In the presence of Mn2+, DNase I recognizes both strands of DNA simultaneously and cleaves them at nearly identical sites.
DNase I can act on single-stranded DNA, double-stranded DNA, chromatin, and RNA:DNA hybrids. It is suitable for RNA extraction, in vitro transcription, and DNA removal in RT-PCR experiments. The production process of this product strictly controls host protein residues, nucleic acid residues, etc., and conforms to GMP production and quality management standards.
Components |
50 KU |
500 KU |
2500 KU |
Storage |
DNase I (50 KU/mL) |
1 mL |
10 mL |
50 mL |
-70°C or below |
Shipping: Dry Ice Shelf life: 2 years |
|