Description:
IC50 Value: 12.17 ± 3.3 uM (Hela cell line)[1]
Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. Solasodine showed selective cytotoxicity against cervical cancer cell line (HeLa) and human myeloid leukemia cell line (U937).
in vitro: Mouse embryonic teratocarcinoma P19 cells exposed to solasodine for 2 days followed by a 5-day washout differentiated into cholinergic neurons that expressed specific neuronal markers and displayed important axonal formation that continued growing even 30 days after treatment [2].
in vivo: A 2-week infusion ofsolasodine into the left ventricle of the rat brain followed by a 3-week washout resulted in a significant increase in bromodeoxyuridine uptake by cells of the ependymal layer, subventricular zone, and cortex that co-localized with doublecortin immunostaining, demonstrating the proliferative and differentiating properties of solasodine on neuronal progenitors. Solasodine treatment in rats resulted in a dramatic increase in expression of the cholesterol- and drug-binding translocator protein in ependymal cells, suggesting a possible role played by neurosteroid production in solasodine-induced neurogenesis. In GAD65-GFP mice that express the green fluorescent protein under the control of the glutamic acid decarboxylase 65-kDa promoter, solasodine treatment increased the number of GABAergic progenitors and neuroblasts generated in the subventricular zone and present in the olfactory migratory tract [2]. intraperitoneal (i.p.) injection of solasodine (25 mg/kg) significantly delayed (p < 0.01) latency of hind limb tonic extensor (HLTE) phase in the PCT-induced convulsions. In the MES model, solasodine significantly reduced (p < 0.001) duration of HLTE at 25, 50, and 100 mg/kg, i.p. in a dose-dependent manner [3]. Oral administration (80 mg/kg body wt/day for 30 days) of solasodine (extracted and isolated from the berries of the Solanum xanthocarpum) to intact dogs significantly decreased the epithelial cell height of cauda epididymides [4].
Toxicity: N/A
Clinical trial: N/A
Chemical Properties
Physical Appearance
A solid
Storage
Store at -20°C
M.Wt
413.64
Cas No.
126-17-0
Formula
C27H43NO2
Synonyms
Purapuridine;Solancarpidine;Solasodin
Solubility
insoluble in DMSO; insoluble in H2O; ≥20.7 mg/mL in EtOH
Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips
We do not recommend long-term storage for the solution, please use it up soon.
Protocol
Cell experiment:[1]
Cell lines
HCT116 cells
Reaction Conditions
20 and 40 μmol/L solasodine for 48 h incubation
Applications
Solasodine significantly inhibited the potential of colony spheroid formation strengthened by TGF-β1 in a concentration-dependent manner. Meanwhile, the mRNA and protein levels of stemness markers including CD133, CD44, Nanog, Oct-4 and Sox-2 were down-regulated with increasing concentrations of solasodine.
Animal experiment:[1]
Animal models
BALB/c/nu/nu nude mice (6 ~ 8 week old, 18 ~ 22 g body weight, half male and half female) xenografted with HCT116 cells
Dosage form
30 and 50 mg/kg
Once daily by intraperitoneal injection for 5 weeks
Applications
Tumor xenografts in both low- (30 mg/kg) and high-dose (50 mg/kg) solasodine groups grew more slowly, and the average volume and weight of the finally resected tumors in solasodine-treated groups were remarkably lower. In harvested tumors, stemness and epithelial-mesenchymal transition (EMT) related molecules were both down-regulated. The results of this study implied that solasodine may be a novel therapeutic drug for human colorectal cancer treatment.
Note
The technical data provided above is for reference only.
References:
1. Zhuang YW, Wu CE, Zhou JY, et al. Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer. Biochemical and Biophysical Research Communications, 2018, 505(2): 485-491.
