[Ser25] Protein Kinase C (19-31)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Protein Kinase C (19-31), (C67H118N26O17), a peptide with the sequence H-ARG-PHE-ALA-ARG-LYS-GLY-SER-LEU-ARG-GLN-LYS-ASN-VAL-OH, MW= 1559.82. this peptide derived from the pseudo-substrate regulatory domain of PKCa (residues 19-31) with a serine at position 25 replacing the wild-type alanine, it was used as protein kinase C substrate peptide for testing the protein kinase C activity.
Protein kinase C also known as PKC, is a family of protein kinase enzymes that are involved in controlling the function of other proteins through the phosphorylation of hydroxyl groups of serine and threonine amino acid residues on these proteins. PKCa has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.
Figure1 Formula of [Ser25] Protein Kinase C (19-31)
Figure2 Signaling pathway of Protein Kinase C
1. Mellor H, Parker PJ (1998). "The extended protein kinase C superfamily". Biochem. J.. 332 ( Pt 2): 281–92
2. Nishizuka Y (1995). "Protein kinase C and lipid signaling for sustained cellular responses" (abstract). FASEB J. 9 (7): 484–96.
3. Vicente Micol. Correlation between Protein Kinase C an Activity and Membrane Phase Behavior. Departamento de Bioquı´mica y Biologı´a Molecular
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥155.9mg/mL in DMSO|
|Chemical Name||[Ser25] Protein Kinase C (19-31)|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|