Recombinant Mouse IFN-gamma
Interferon-γ (IFN-γ), also known as type II interferon or immune interferon, is a cytokine produced primarily by T lymphocytes and natural killer cells. This protein has no significant homology to IFN-β or various IFN-α family proteins. Mature IFN-γ exists as a non-covalently linked homodimer. It shares high-sequence identity with rat IFN-γ (86%). IFN-γ was initially characterized based on its antiviral activity. The protein also exerts antiproliferative, immunomodulatory, and pro-inflammatory activities, making it important in host defense mechanisms. IFN-γ induces cytokine production and upregulates the expression of class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. It regulates macrophage effector function; Affects isotype switching and enhances B cell secretion of immunoglobulins. In addition, IFN-γ enhances TH1 cell expansion and may be required for TH1 cell differentiation.
Reference
1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol,
2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.
3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.
4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.
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Gene ID |
15978 |
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Accession # |
P01580 |
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Alternate Names |
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma |
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Source |
Escherichia coli. |
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Tag |
His, Strep |
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M.Wt |
15.6 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 0.3-0.9 ng/mL. Measured in an anti-viral assay using L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity: > 95 % by SDS-PAGE.
- Datasheet
Endotoxin: Less than 1 EU/µg as determined by LAL method.