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One-step TUNEL Cy3 Apoptosis Detection Kit

Catalog No.
K1134
TUNEL Cy3 Apoptosis Detection
Grouped product items
SizePriceStock Qty
20rxns
$220.00
In stock
50rxns
$320.00
In stock
100rxns
$500.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Description

The One-step TUNEL Cy3 Apoptosis Detection Kit can be used to detect breaks in nuclear DNA in tissues or cells during apoptosis. Intracellular DNA endonucleases are activated during apoptosis, cutting off genomic DNA between nucleosomes, and DNA degrades into 180 - 200 bp or other integer multiple fragments. The 3 ́-OH terminal produced by the genomic DNA break is added to the end with the labeled dUTP at its end under the action of terminal Deoxynucleotidyl Transferase (TdT), This allows detection under a fluorescence microscope or flow cytometry (Ex/Em=550nm/570nm).

This kit can be used in a wide range of applications to detect apoptosis in frozen or paraffin sections, as well as to detect apoptosis of cultured adherent or suspension cells.

Product Citation

Features

293A cells were divided into three groups: control group, Positive group using DNase I treatment, Apoptosis group using 10μM Camptothecin treatment. After 12 hours of treatment, TUNEL kit (K1134) was used to detect cell Apoptosis.

Quality Control

Quality Control & DataSheet

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Related Biological Data

One-step TUNEL Cy3 Apoptosis Detection Kit
 

Components and Storage

Place product at -20 °C for storage, stable for 1 year; Among them, Cy3-dUTP Labeling Mix, stored at -20°C protected from light.