One-step TUNEL Cy3 Apoptosis Detection Kit

The One-step TUNEL Cy3 Apoptosis Detection Kit can be used to detect breaks in nuclear DNA in tissues or cells during apoptosis. Intracellular DNA endonucleases are activated during apoptosis, cutting off genomic DNA between nucleosomes, and DNA degrades into 180 - 200 bp or other integer multiple fragments. The 3 ́-OH terminal produced by the genomic DNA break is added to the end with the labeled dUTP at its end under the action of terminal Deoxynucleotidyl Transferase (TdT), This allows detection under a fluorescence microscope or flow cytometry (Ex/Em=550nm/570nm).

This kit can be used in a wide range of applications to detect apoptosis in frozen or paraffin sections, as well as to detect apoptosis of cultured adherent or suspension cells.

Assessment of apoptosis in vitro and in vivo using the TUNEL Kit.​ (A) HeLa cells were treated with or without DNase I (positive control) prior to TUNEL staining using the TUNEL Kit (Cat# K1134). (B) C57BL/6J mice were randomized into two groups: control (saline) and experimental (20 mg/kg cisplatin), with administration via intraperitoneal injection. Mice were sacrificed 24-48 h post-injection, and kidney tissue sections were prepared for TUNEL staining (Cat# K1134) to evaluate apoptotic levels.

Place product at -20 °C for storage, stable for 1 year; Among them, Cy3-dUTP Labeling Mix, stored at -20°C protected from light.