Mito-Tracker Green

Mito-Tracker Green is a green-fluorescent mitochondria probe that selectively labels mitochondria. Some conventional fluorescent probes for mitochondria such as tetramethylrosamine and rhodamine 123, are sequestered by functional mitochondria. These probes are easily washed out of cells upon loss in the mitochondria membrane potential. This characteristic limits the use in experiments that require regents that affect the function of the mitochondria. To overcome the limitation, it is better to use Mito-Tracker Green which labels mitochondria regardless of mitochondrial membrane potential. In addition, Mito-Tracker Green stains live cells well but is not well-retained after fixation, so it is not suitable for fixed cell staining.

Figure 1. Super-resolution microscopy image of U2OS cells stained with 100 nM Mito-Tracker Green (B8811) for 30 min.