Liproxstatin-1
Liproxstatin-1 (CAS 950455-15-9) is a potent inhibitor of ferroptosis, an iron-dependent, non-apoptotic mode of regulated cell death characterized by lipid peroxidation. It inhibits ferroptosis with an IC50 of approximately 22 nM. Mechanistically, Liproxstatin-1 prevents lipid peroxidation induced by small-molecule ferroptosis inducers (FINs), such as RSL3, notably in GPX4-deficient cellular systems. In animal models, Liproxstatin-1 mitigates ferroptosis-induced tissue injury, prolonging survival in mice with conditional kidney-specific Gpx4 deletion and reducing organ damage in hepatic ischemia/reperfusion injury models. This compound is thus valuable for studying ferroptosis-related cell death in renal and hepatic pathological conditions.
- 1. Nicole L Jenkins, Simon A James, et al. "Changes in ferrous iron and glutathione promote ferroptosis and frailty in aging Caenorhabditis elegans." Elife. 2020 Jul 21;9:e56580. PMID: 32690135
- 2. Xiaohong Zhang, Xueshuang Xing, et al. "Ionizing radiation induces ferroptosis in granulocyte-macrophage hematopoietic progenitor cells of murine bone marrow." Int J Radiat Biol , 1-35 2020 Jan 6. PMID: 31906761
- 3. Nicole L. Jenkins, Simon A. James, et al. "Ferrous-glutathione coupling mediates ferroptosis and frailty in Caenorhabditis elegans." bioRxiv. 2019 March 31.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 340.85 |
| Cas No. | 950455-15-9 |
| Formula | C19H21ClN4 |
| Solubility | insoluble in H2O; ≥10.5 mg/mL in DMSO; ≥2.39 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | N-(3-chlorobenzyl)-4'H-spiro[piperidine-4,3'-quinoxalin]-2'-amine |
| SDF | Download SDF |
| Canonical SMILES | ClC1=CC(CNC(C2(CCNCC2)N3)=NC4=C3C=CC=C4)=CC=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Gpx4-/- cells |
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Preparation method |
The solubility of this compound in DMSO is > 10.5 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
72 hrs |
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Applications |
Liproxstatin-1 inhibited the growth of Gpx4-/- cells with an IC50 value of 22 nM. At the dose of 50 nM, Liproxstatin-1 completely prevented lipid peroxidation. Liproxstatin-1 (200 nM) dose-dependently protected Gpx4-/- cells against ferroptosis-inducing agents, such as L-buthionine sulphoximine (10 μM), erastin (1 μM) and RSL3 (0.5 μM), whereas it failed to rescue cell death caused by staurosporine (0.2 μM) and H2O2 (200 μM). |
| Animal experiment [1]: | |
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Animal models |
GreERT2; Gpx4fl/fl mice |
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Dosage form |
10 mg/kg; i.p. |
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Applications |
In GreERT2; Gpx4fl/fl mice, Liproxstatin-1 significantly extended the survival period. The TUNEL staining results after 9-day treatment showed a markedly reduced number of TUNEL+ cells in the Liproxstatin-1 treatment group than in the vehicle control group, indicating that Liproxstatin-1 delayed ferroptosis in tubular cells. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Friedmann Angeli JP, Schneider M, Proneth B, et al. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice. Nat Cell Biol, 2014, 16(12): 1180-1191. |
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Quality Control & MSDS
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Chemical structure
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Related Biological Data
