HyperScript™ III RT SuperMix for qPCR (with gDNA wiper)

HyperScript™ III Reverse Transcriptase is a third-generation reverse transcriptase based on M-MLV Reverse Transcriptase that has been genetically engineered. HyperScript™ III Reverse Transcriptase generates a reduced RNase H activity and improves thermal stability and fidelity. In addition, it has characteristics of higher cDNA synthesis yield and longer length, higher reverse transcription efficiency for high-GC content RNA. HyperScript™ III Reverse Transcriptase has an enhanced affinity for templates and is suitable for reverse transcription of small amounts of template as well as low-copy genes, you can use PCR to amplify cDNA up to 12.3 kb.

The HyperScript™ III RT SuperMix for qPCR (with gDNA wiper) is suitable for two-step qRT-PCR assays, 5 × HyperScript™ III SuperMix contains all components for reverse transcription except the template. The inclusion of the 4× gDNA wiper in the product is intended to remove potential genomic DNA contamination before reverse transcription. This kit is ideal for reverse transcription of low-concentration RNA templates. The 5X HyperScript™ III SuperMix doesn’t freeze at -20°C and is easy to use.

This product has been optimized for qPCR, especially the ratio of Oligo (dT)23VN primers /Random primers, the cDNA synthesis can be initiated from all regions of the RNA transcript with the same efficiency, ensuring maximum authenticity and reproducibility of qPCR results. The reverse transcription products can be employed in SYBR Green or probe-based qPCR, you can choose appropriate reagents for gene expression analysis according to your purpose.