Fluorometric Monoamine Oxidase Assay Kit

Monoamine oxidases (MAOs) are flavoproteins mainly distributed in the outer mitochondrial membrane that catalyze the oxidation of amine substrates to produce hydrogen peroxide and ammonia. MAOs are divided into two isoenzymes, MAO-A and MAO-B, and although they share up to 70% sequence similarity, there are significant differences in cell distribution, substrate specificity, and function. MAO-A is mainly distributed in catecholaminergic neurons; Whereas, MAO-B is mainly found in astrocytes and acts on substrates such as dopamine. Studies have shown that the abnormal activation of MAOs is closely related to a variety of neuropsychiatric diseases, such as Alzheimer's disease and Parkinson's disease.

Fluorometric Monoamine Oxidase Assay Kit can be used to rapidly detect the MAOs activity. The detection principle is based on the hydrogen peroxide, a by-product of MAO catalyzed amine substrates such as tyramine. In this process, in the presence of HRP, Hydrogen Peroxide reacts with a specific fluorescent probe, Amplex Red, to generate a highly fluorescent resorufin. The fluorescence intensity is proportional to the activity of MAOs. At the same time, the kit provides MAO-A and MAO-B inhibitors, which can detect the total MAO activity as well as MAO-A and MAO-B isoenzyme activities.

Figure 1: Hydrogen Peroxide standard curve

Figure 2: Resorufin standard curve

Figure 3: MAO-T, MAO-A, MAO-B activity in mouse serum samples