EZ Cap™ Fluc-EGFP mRNA (m1Ψ)
EZ Cap™ Fluc-eGFP mRNA (m1Ψ) is a ready-to-use mRNA product that co-expresses enhanced green fluorescent protein (EGFP) and firefly luciferase (Fluc). EGFP is a commonly used reporter molecule that emits strong, bright green fluorescence upon excitation. The Fluc gene is derived from the firefly Photinus pyralis. This enzyme catalyzes the ATP-dependent oxidation of D-luciferin, producing chemiluminescence at approximately 560 nm. It is suitable for research applications including mRNA delivery, translation efficiency, cell viability, and in vivo imaging.
Key features:
Cap1 Structure: The mRNA incorporates a Cap1 analog at the 5' end, ensuring high translation initiation efficiency, improved stability, and reduced innate immune recognition, leading to stronger and more sustained luciferase expression.
Modified Nucleotides: It contains N1-Methylpseudo-UTP modified nucleotides, which decreases immunogenicity, enhances mRNA stability, and increases translational efficiency, resulting in more reliable and reproducible protein yield.
poly(A) tail: It contains an optimized poly(A) tail of approximately 100 nucleotides. This specific length is engineered to maximize transcript stability by resisting degradation and to synergize with the 5' cap for superior, sustained translation efficiency, ensuring robust protein yield in your applications.
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mRNA Length |
2908 nucleotides |
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Concentration |
~1 mg/mL |
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Excitation max (nm) |
488 nm (EGFP) |
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Emission max (nm) |
509 nm (EGFP) |
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Buffer |
1 mM Sodium Citrate, pH 6.4 |
Storage |
-40°C or below |
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General tips |
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
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Shipping Condition |
ship with dry ice |
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