E. coli DNA ligase

E. coli DNA ligase is a recombinant enzyme purified from Escherichia coli strains, carrying the gene of E. coli DNA ligase. E. coli DNA ligase catalyzes the formation of phosphodiester bonds between the 5' phosphate and the 3' hydroxyl of adjacent duplex DNA strands, with very low activity for blunt-ended DNA. The enzyme is most effective in Tris-HCl buffer at pH 7.5-8.0 and requires NAD+ as a cofactor. This enzyme is mainly employed for nick sealing and cohesive DNA end ligation. E. coli DNA ligase remains active within a temperature range of 4-37℃.