dUTPase
dUTP pyrophosphatase (dUTPase) is a key enzyme in DNA synthesis and widely distributed in various kinds organisms such as eukaryotes, prokaryotic cells, and viruses. The enzyme hydrolyzes dUTP to dUMP and inorganic pyrophosphate (PPi) in the cytoplasm, thereby minimizing the misinsertion of uracil in DNA synthesis, reducing the ratio of dUTP/dTTP in cells, preventing incorporation into DNA strands, and maintaining the fidelity and smooth progress of genome replication. At the same time, dUMP, a hydrolysate product of dUTP, is the substrate of TS and is a precursor raw material for the biosynthesis of dTTP in vivo.
This product is an optimized formation of heat stable dUTPases that maximize high-fidelity PCR efficiency, remove contaminated dUTPs in PCR reactions and dNTP solutions, and reduce the chance of mutation. dUTPase increases the yield, length, and fidelity of PCR products, enabling further downstream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications, such as cloning and subsequent recombinant protein techniques, as well as gene expression analysis (semi-quantitative RT-PCR technology and real-time PCR analysis) where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is dUTP-specific and critical to the fidelity of DNA replication and repair.
The detailed parameters of dUTPase are as follows:
|
Name |
dUTPase |
|
Biological activity |
103 U/μg |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
State |
Liquid |
|
Formulation |
dUTPase is supplied in 20 mM Tris-HCl (pH 8.2), 1 mM DTT, 0.1 mM EDTA, 100mM KCl, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at a concentration of 1000 U/µL of the enzyme. |
|
Concentration |
1 mg/mL |
|
Sequence |
The sequence cannot be shared. |
Table 1 Detailed parameters of dUTPase.
| Components | K1108-10,000 U | K1108-50,000 U |
| dUTPase (1 mg/mL) | 10 μL | 50 μL |
| Storage buffer | 1 mL | 5 mL |
Store the components at -20°C for 12-18 months. | ||