Dil
DiI, also called DiIC18(3), is an orange fluorescent probe that is widely used as a lipophilic tracker in living and fixed tissues and cells. DiI can insert into the membrane, and diffuse rapidly, staining the entire membrane surface orange. DiI usually does not affect cell viability and development, so it is commonly used in cell tracking, such as anterograde and retrograde neuronal tracking. DiI has remained viable for up to four weeks in culture and up to one year in vivo.
DiI staining can be used in conjunction with immunofluorescence. It is recommended to use formaldehyde (PFA) for fixation after DiI staining. Meanwhile, if permeabilization is required, it is suggested to use Triton X-100 or digitonin. But permeabilization may affect the localization of DiI in the cell membrane. In addition, DiI has many other applications including detecting cell-cell fusion and adhesion, tracking cell migration, and labeling lipoproteins.
| Storage | Store at -20°C, away from light |
| M.Wt | 933.89 |
| Cas No. | 41085-99-8 |
| Formula | C59H97ClN2O4 |
| Synonyms | DiIC18(3) |
| Solubility | ≥55.7 mg/mL in DMSO; ≥5.64 mg/mL in EtOH with ultrasonic; insoluble in H2O |
| Chemical Name | 2-((E)-3-((E)-3,3-dimethyl-1-octadecylindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-1-octadecyl-3H-indol-1-ium perchlorate |
| SDF | Download SDF |
| Canonical SMILES | CCCCCCCCCCCCCCCCCC[N+]1=C(/C=C/C=C2C(C)(C)C(C=CC=C3)=C3N\2CCCCCCCCCCCCCCCCCC)C(C)(C)C4=C1C=CC=C4.O=Cl(=O)([O-])=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
| Components | B8804-10 mg | B8804-25 mg |
| DiI | 10 mg | 25 mg |
The product should be stored at -20°C away from light and moisture. Stable for 1 year. The stock solution should be stored at -20°C away from light. Stable for 6 months. | ||