ATP Assay Kit (Colorimetric/Fluorometric)

ATP is the most crucial energy source within cells, playing a vital role in various cellular processes. Abnormal changes in ATP levels are often associated with impaired cellular functions, making the detection of ATP levels highly important.

This kit provides a simple and sensitive method for quantifying ATP in tissues, cells, and other samples. Glycerol kinase can phosphorylate glycerol using ATP as the phosphate donor, producing glycerol-3-phosphate. Subsequently, glycerol-3-phosphate is oxidized by glycerol phosphate oxidase, generating hydrogen peroxide (H₂O₂). In the presence of horseradish peroxidase, the produced H₂O₂ reacts with the highly sensitive Amplex Red probe to form the fluorescent red product, resorufin. The fluorescence intensity and absorbance of resorufin are directly proportional to the ATP concentration in the sample. Consequently, this kit offers flexible detection, allowing measurements by either colorimetric or fluorometric methods.

Figure 1. Typical standard curve as determined by colorimetric (left) or fluorometric (right) methods.