5X DNA annealing buffer

DNA Annealing Buffer is designed for conventional DNA oligo annealing. It effectively prevents hairpin structure formation of single DNA oligos, and the annealed double-stranded oligos can be directly used for ligation with restriction enzyme-digested and purified plasmids. The protocol is simple: mix the DNA oligos with 5X DNA Annealing Buffer at a proper ratio, place in a PCR thermal cycler, and the annealing reaction will be completed in approximately 90 minutes.