Z-DEVD-AFC
Z-DEVD-AFC is a fluorometric substrate for caspase-3 (Km = 9.7µM) and other related caspases. The sequence DEVD is based on PARP cleavage at Asp216 for caspase-3. During apoptosis, caspase-3 cleaves the substrate and release AFC, thus the caspase activity can be calculated by the fluorescent detection of AFC at Ex. = 400 nm and Em. = 505 nm, or by a blue to green shift in fluorescence. Z-DEVD-AFC can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
References:
1. Jiuru Sun.; Stephen P. Bottomley.; Sharad Kumar.; Phillip I. Bird. Recombinant Caspase-3 Expressed inPichia pastorisIs Fully Activated and Kinetically Indistinguishable from the Native Enzyme. Biochemical and Biophysical Research Communications. 1997, 238 (3), 920-924.
2. Kieran F. Harvey.; Natasha L. Harvey.; Julie M. Michael.; Gayathri Parasivam.; Nigel Waterhouse.; Emad S. Alnemri?.; Dianne Watters.; Sharad Kumar. Caspase-mediated Cleavage of the Ubiquitin-protein Ligase Nedd4 during Apoptosis. The Journal of Biological Chemistry. 1998, 273 13524-13530.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 821.71 |
| Cas No. | 1135416-11-3 |
| Formula | C36H38F3N5O14 |
| Solubility | ≥82.2 mg/mL in DMSO; insoluble in H2O; ≥2.5 mg/mL in EtOH with ultrasonic |
| Chemical Name | 8-(2-carboxyethyl)-5-(carboxymethyl)-11-isopropyl-3,6,9,12-tetraoxo-14-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(OCC1=CC=CC=C1)=O)CC(O)=O)=O)C(NC(C(C)C)C(NC(CC(O)=O)C(NC2=CC(O3)=C(C=C2)C(C(F)(F)F)=CC3=O)=O)=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
