Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit
Xanthine oxidase (XO) is a kind of xanthine oxidoreductase that generates reactive oxygen species. XO is present in the jejunum and liver in healthy individuals. However, XO is released into circulation in various liver disorders. Measurement of serum XO level is a sensitive indicator of acute liver damage such as jaundice.
The Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit provides a sensitive, simple, fast and convenient way for accurate detection of XO activity in various samples based on colorimetric and fluorometric method. In the assay, XO oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with the Probe to yield fluorescence (at Ex/Em = 535/587 nm) and color (at λ = 570 nm). The fluorescence intensity or color generated is proportional to XO content. The kit can detect 1-100 mU xanthine oxidase in 100 µL reaction volume.
| Features | Fast and convenient; Simple procedure; Takes approx. less than 40 minutes; Sensitive assays for measuring xanthine oxidase activity in various biological samples. |
| Shipping | Blue Ice |
| Storage Conditions | Store at -20°C. |
| Kit components | XO Assay Buffer, OxiRed™ Probe (in DMSO), XO Enzyme Mix, XO Substrate Mix, XO Positive Control, H2O2 Standard (0.88 M) |