V-9302

V-9302 (CAS No.: 1855871-76-9) is a synthetic small-molecule tool compound for biomedical research and drug discovery. As a competitive antagonist of transmembrane glutamine flux, it selectively inhibits the neutral amino acid transporter ASCT2 (SLC1A5), with minimal effect on ASCT1 activity; in heterologous expression systems and mammalian cells (such as HEK-293), it can inhibit ASCT2-mediated glutamine uptake, with in vitro activity in the low micromolar range. Reported half-maximal inhibitory activity for the human and rat transporters is approximately 9–10 micromolar, and it reduces cell viability in multiple glutamine-dependent tumor cell lines at similar low micromolar effective concentrations; in specific experimental contexts (such as Xenopus laevis oocytes and certain tumor cell models), inhibition of transporters such as SNAT2 (SLC38A2) and LAT1 (SLC7A5) has also been observed, suggesting that context-dependent multi-target effects may exist among amino acid transporters; by limiting cellular uptake of glutamine, this compound is commonly used to analyze tumor metabolic dependency, amino acid transport physiology, and transporter pharmacology, and to support target validation and mechanism-oriented screening studies. Typical applications include cell-level functional and metabolic phenotyping assays, transporter-family profiling platform evaluation, and exploratory in vivo studies (such as mouse xenograft models) to monitor changes in glutamine uptake at the tissue level; practical observations during experimental handling indicate that when diluted with phosphate-buffered saline, a clear aqueous phase can transform into a uniform suspension system; this product is intended only for laboratory research and method development, not for human or animal ingestion or any non-research use. The specific concentration or dosing regimen used in experiments is typically determined by the research objective and experimental design.