Universal Powerful Antigen Retrieval Solution(10X)

Fixation of cells or tissues with paraformaldehyde, formaldehyde, or other aldehyde-based reagents induces cross-linking between proteins, which masks antigenic sites in the sample and leads to reduced staining signals or even false-positive results in immunostaining. Antigen Retrieval Solution is used for antigen retrieval in aldehyde-fixed samples such as paraffin-embedded sections and frozen sections, effectively removing aldehyde-induced protein cross-links and fully exposing antigenic epitopes in samples like paraffin sections, thereby greatly improving immunostaining results.

Antigen retrieval is typically required for paraffin sections to enhance immunostaining results. For frozen sections, antigen retrieval is optional; however, many studies have shown that it significantly improves staining outcomes, especially when immunostaining of frozen sections is suboptimal. In principle, for any sample fixed with paraformaldehyde, formaldehyde, or other aldehyde-based reagents—whether frozen sections or cell smears—antigen retrieval effectively removes protein cross-links and fully exposes antigenic epitopes, thereby greatly improving immunostaining results.

This product is an antigen retrieval solution that directly reverses cross-linking caused by aldehyde-based fixatives. Its antigen retrieval efficiency is generally superior to that of commonly used citrate-based antigen retrieval solutions. It is suitable for paraffin sections and can also be used for frozen sections and other sample types.