Recombinant Mouse CD4, Tag Free
In molecular biology, CD4 (cluster of differentiation 4) is a glycoprotein that functions as a co-receptor for the T cell receptor (TCR). CD4 is found on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells. CD4? helper T cells are a type of white blood cell that is an important component of the human immune system. CD4 is still expressed in most tumors derived from helper T cells. Therefore, most peripheral T-cell lymphomas and associated malignancies can be identified by CD4 immunohistochemistry of tissue biopsy samples. This antigen has also been associated with a variety of autoimmune diseases, such as vitiligo and type 1 diabetes. T cells also play an important role in autoinflammatory diseases. It is helpful to quantify the number of T cells in fresh frozen tissue using CD4?, CD8?, and CD3? T cell markers when testing drug efficacy or studying disease.
| Gene ID | 12504 |
| Accession # | NP-038516.1 |
| Alternate Names | rMuCD4 |
| Source | HEK293 |
| Protein sequence | KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT |
| M.Wt | The protein has a calculated MW of 41.2 KDa |
| Appearance | Solution protein. |
| Stability & Storage | Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 3 years from date of receipt, -20 to -70 °C as supplied. |
| Concentration | 1 mg/mL |
| Formulation | Supplied as a 0.2 μm filtered solution in PBS, pH7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
| Biological Activity | Fully biologically active as determined by the ability of the immobilized protein to support the adhesion of Jurkat human T lymphocyte cells. The EC50 for this effect is 2.9 μg/mL. |
| Shipping Condition | Shipping with dry ice. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 95 % by SDS-PAGE
- Datasheet
Endotoxin: Less than 1.0 EU/μg as determined by LAL method.