Recombinant Human Eotaxin/CCL11
Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Human CCL11 is approximately 63 % identical at the amino acid level to murine CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.
Reference
1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12
2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7
3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56
4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.
| Gene ID | 6356 |
| Accession # | P51671 |
| Alternate Names | Small-inducible cytokine A11 |
| Source | Escherichia coli. |
| M.Wt | Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| AA Sequence | GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuEotaxin/CCL11 as determined by LAL method.