Recombinant Human BMP-7
Bone Morphogenetic Protein 7 is one of the BMPs, some of which belong to the TGF-beta superfamily (BMP2-7). There are more than thirteen BMPs have been discovered nowadays and they are involved in inducing cartilage and bone formation. BMP-7 is mainly expressed in kidney and bladder. It is also present in developing eyes, brain and ear during embryogenesis. BMP-7 also named osteogenic protein-1 (OP-1) is a potent osteoinductive cytokine and plays role in osteoblast differentiation, SMAD1 production and renal development and repair. Human BMP-7 is synthesized with a signal sequence (29 a.a.), a propeptide (263 a.a.), and a growth factor domain (139 a.a.). The growth factor domain of human BMP-7 shares 98 % a.a. sequence identity with mouse and rat BMP-7.
Reference:
1. Zeisberg M, Hanai J, Sugimoto H, et al. 2003. Nat Med, 9: 964-8.
2. Phillips FM, Turner AS, Seim HB, 3rd, et al. 2006. Spine J, 6: 500-6.
3. Veerasamy M, Nguyen TQ, Motazed R, et al. 2009. Am J Physiol Renal Physiol, 297: F1238-48.
4. Pauly S, Klatte F, Strobel C, et al. 2012. J Shoulder Elbow Surg, 21: 464-73.
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Gene ID |
655 |
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Accession # |
P18075 |
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Alternate Names |
OP-1 |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids. |
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AA Sequence |
STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Data is not available. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & MSDS
- View current batch:
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Purity > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuBMP-7 as determined by LAL method.