Recombinant Human Betacellulin
Betacellulin (BTC) is a member of the EGF family of cytokines that also includes EGF, TGF-a, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. It is expressed in most tissues including kidney, uterus, liver and pancreas. BTC also presents in body fluids, including serum, milk, and colostrum. At the amino acid sequence level, human mature BTC protein exhibits 80 % identity with mouse BTC protein. The protein binds to EGFR, ERBB4 and other EGF receptor family members and acts as a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Reference
1. Pinkas-Kramarski R, Lenferink AE, Bacus SS, et al. 1998. Oncogene, 16: 1249-58
2. Dunbar AJandGoddard C. 2000. Int J Biochem Cell Biol, 32: 805-15
3. Mifune M, Ohtsu H, Suzuki H, et al. 2004. Am J Physiol Cell Physiol, 287: C807-13
4. Schneider MR, Mayer-Roenne B, Dahlhoff M, et al. 2009. J Bone Miner Res, 24: 455-67.
| Gene ID | 685 |
| Accession # | P35070 |
| Source | Escherichia coli. |
| M.Wt | Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein. |
| AA Sequence | DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuBTC as determined by LAL method.